Part:BBa_K277088

3L.3_23.C3.09

3L.3_23.C3.09 is 829 bases long and is cloned into the pGem-T vector.

3L.3_23.C3.09 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. The whole synthetic chromosome may be viewed at http://macbeth.clark.jhu.edu/cgi-bin/gbrowse 3L.3_23.C3.09 is a constituent of 3L.3_23.C3 (along with 3L.3_23.C3.01, 3L.3_23.C3.02, 3L.3_23.C3.03, 3L.3_23.C3.04, 3L.3_23.C3.05, 3L.3_23.C3.06, 3L.3_23.C3.07, 3L.3_23.C3.08, 3L.3_23.C3.10, 3L.3_23.C3.11, 3L.3_23.C3.12, 3L.3_23.C3.13, and 3L.3_23.C3.14.)

This part contains at least part of the following features (positions offset from first base of sequence):

kind and name offset notes

gene YCL026C-B (-2..579) Putative protein of unknown function%3B green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus

mutation_affecting_coding_sequence YCL026C-B_re_remove_BglI (166..177) removal of BglI

reverse_primer YCL026C-B_tagr1v1 (295..322)

forward_primer YCL026C-B_tagf1v1 (91..118)

Sequence (corresponds to coordinates 64121..64949 in synthetic chromosome yeast_chr3_3_23)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 318
  Illegal XhoI site found at 730
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]